Capto MMC and Capto S HiScreen columns (4.7 ml, 0.77 × 10 cm) were purchased from GE Healthcare (Uppsala, Sweden) for the lab scale study.

HiTrap™ and HiScreen™ prepacked columns can be utilized for confirming screening and optimization results in packed bed conditions, as well as for convenient small scale purification. Capto MMC and Capto S resins were purchased from GE Healthcare (Uppsala, Sweden) for the microplate study. With its high dynamic binding capacity and high selectivity, Capto L increases process economy and process design flexibility.Ĭapto L is a BioProcess™ medium supported for commercial manufacturing and enables a high-productivity platform approach to antibody fragment purification.Īvailability of flexible formats such as PreDictor™ plates and PreDictor RoboColumn™ units speeds up the screening and optimization of binding and elution conditions. Capto adhere is a multimodal ion exchanger and is designed to be used as a second or third step in monoclonal antibody (MAb) purification processes, (i.e., after capture on a. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein L ligand, which has strong affinity for the variable region of antibody’s kappa light chain.Ĭapto L is therefore suitable for purification of a range of antibody fragments such as Fabs, single-chain variable fragments and domain antibodies. GE Healthcare Life Sciences Instructions 28-9064-05 AC Affinity Chromatography Capto adhere Capto adhere is a multimodal BioProcess medium for intermediate. GE Healthcare’s Capto L affinity chromatography medium (resin) is designed for the purification of antibody fragments.

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